Carver Center for Genomics - Sequencing Center

Sanger or dideoxy sequencing has remained the most commonly used DNA sequencing technique up until recently, and was instrumental in sequencing genomes of model organisms including Humans, Drosophila and Arabidopsis. However, with advances made in the fields of microfluidics, nanotechnology and informatics, nowadays, alternate sequencing technologies also known as the 'NextGen' sequencing technologies are gaining popularity. The term 'NextGen' sequencing is used to collectively describe technologies other than Sanger sequencing that have the capability of producing increased sequencing depth and coverage at a fraction of time, labor, and cost. Currently, these technologies include the 454 GS20 pyrosequencing technology (Roche Applied Science), Genome Analyzer IIx (Illumina, Inc.), SOLiD (Applied Biosystems), and the Heliscope Single Molecule Sequencer (Helicos, Inc.). In addition to Sanger sequencing, since December 2008, CCG has initiated facilitation of next generation sequencing projects. We have completed over a dozen 454 sequencing projects and several other deep sequencing projects are in the pipeline.

Illumina Sample Queue

1/26/2012 - Currently, we have samples occupying 2 lanes for 1x36 bp or 1x75 bp sequencing.