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Dailey Lab Movies
 


Microglia

Microglia

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Microglial phagocytosis of naturally apoptotic cells in layer II of the developing (P7) mouse neocortex.Microglia are green by virtue of GFP expression (CX3CR1 heterozygous mice). Apoptotic cells (red) are labeled with PSVue550, a phosphatidylserine-binding dye.

Movie by Mike Dailey.


Microglia

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3-D time-lapse sequence (2 MB QuickTime file) showing activation of microglia in a neonatal mouse hippocampal brain slice.  Microglia were labeled with Alexa488-IB4.  Use red-green (or red-blue) stereo glasses (red over left eye) to view 3D. Total sequence time is ~6 hr.

Movie by Dana Kurpius.



Microglia

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Time-lapse sequence (4.8 MB QuickTime file) showing motile and locomotory microglia moving among dead cells in a rat hippocampal brain slice culture (P6 + 2 DIV).  Microglia were labeled with FITC-IB4 (green) and dead cell nuclei were labeled with To-Pro-3 (red).  A confocal microscope was used to collect images in two fluorescent channels simultaneously.  Total sequence time is 5 hr.

Movie by Mark Petersen. (See Petersen & Dailey, 2004. Fig. 4)



Microglia

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Higher magnification view (2.9 MB QuickTime file) from the same experiment showing dynamics of microglial motility.  Note a mitotic microglial cell (lower left of center) near the beginning of the time sequence.  Also, near the end of the time sequence, a microglial cell sweeps in from the upper left corner and picks up a dead cell nucleus "on the fly".

Movie by Mark Petersen. (See Petersen & Dailey, 2004, Fig. 4)



Microglia

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Microglia rapidly engulf cells that die spontaneously in brain slice cultures (1.6 MB QuickTime file).  Several Sytox Orange-labeled dead cell nuclei (yellow balls) are already apparent at the start of this movie.  When a cell dies in the presence of a dead cell marker, its nucleus becomes labeled.  In this movie, a dead cell nucleus appears spontaneously in the bottom right hand corner of the image.  Within minutes, one of the microglial cells extended a long process directly toward the dead cell nucleus, engulfed it, and dragged it away.  This demonstrates that activated microglia can sense and respond very rapidly to cell death events in brain tissues.  Rapid clearance may reduce secondary tissue damage due to release of toxic products from dead cells.  Tissue is P6 + 3 DIV.

Movie by Mark Petersen. (See Petersen & Dailey, 2004, Fig. 6)


Astrocytes

Three-dimensional reconstruction of an astrocyte

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Astrocytes in rat hippocampal slice cultures develop a complex 3-D morphology. This movie (840K AVI file) shows a three-dimensional reconstruction of a rat astrocyte expressing LCK-GFP.  A confocal microscope was used to image the cell in a cultured hippocampal slice.

Movie by Adrienne Benediktsson and Ryan Jeffrey.