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 Click image to play |
Microglial phagocytosis of naturally apoptotic cells in layer II of the developing (P7) mouse neocortex.Microglia are green by virtue of GFP expression (CX3CR1 heterozygous mice). Apoptotic cells (red) are labeled with PSVue550, a phosphatidylserine-binding dye. Movie by Mike Dailey. |
 Click image to play |
3-D time-lapse sequence
(2 MB QuickTime file) showing activation of microglia in a neonatal
mouse hippocampal brain slice. Microglia
were labeled with Alexa488-IB4. Use red-green (or red-blue)
stereo glasses (red over left eye) to view 3D. Total sequence time
is ~6 hr. Movie by Dana Kurpius. |
 Click image to play |
Time-lapse sequence
(4.8 MB QuickTime file) showing motile and locomotory microglia moving
among dead cells in a rat hippocampal brain slice culture (P6 + 2
DIV). Microglia were labeled with
FITC-IB4 (green) and dead cell nuclei were labeled with To-Pro-3 (red).
A confocal microscope was used to collect images in two fluorescent
channels simultaneously. Total sequence time is 5 hr. Movie by Mark Petersen. (See Petersen & Dailey, 2004. Fig. 4) |
 Click image to play |
Higher magnification
view (2.9 MB QuickTime file) from the same experiment showing dynamics
of microglial motility. Note a mitotic
microglial cell (lower left of center) near the beginning of the time
sequence. Also, near the end of the time sequence, a microglial
cell sweeps in from the upper left corner and picks up a dead cell
nucleus "on the fly". Movie by Mark Petersen. (See Petersen & Dailey, 2004, Fig. 4) |
 Click image to play |
Microglia rapidly engulf
cells that die spontaneously in brain slice cultures (1.6 MB QuickTime
file). Several Sytox Orange-labeled
dead cell nuclei (yellow balls) are already apparent at the start
of this movie. When a cell dies in the presence of a dead cell
marker, its nucleus becomes labeled. In this movie, a dead cell
nucleus appears spontaneously in the bottom right hand corner of the
image. Within minutes, one of the microglial cells extended
a long process directly toward the dead cell nucleus, engulfed it,
and dragged it away. This demonstrates that activated microglia
can sense and respond very rapidly to cell death events in brain tissues.
Rapid clearance may reduce secondary tissue damage due to release
of toxic products from dead cells. Tissue is P6 + 3 DIV. Movie by Mark Petersen. (See Petersen & Dailey, 2004, Fig. 6) |
 Click image to play |
Astrocytes in rat hippocampal slice
cultures develop a complex 3-D morphology. This movie (840K AVI
file) shows a three-dimensional reconstruction of a rat astrocyte
expressing LCK-GFP. A confocal microscope was used to image
the cell in a cultured hippocampal slice. Movie by Adrienne Benediktsson and Ryan Jeffrey. |
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Copyright © 2004 All rights reserved |
