I am interested in understanding the neural mechanisms that regulate circadian rhythms, the daily patterns of physiology and behavior that are prominent in many species. My lab studies the model organism Drosophila melanogaster, which exhibits robust daily rhythms in several behaviors, including locomotor activity (Figure 1). Genetic approaches in Drosophila have led to the identification of a number of key circadian rhythms genes, including several with conserved function in mammals.
Much of the focus in circadian rhythms research has gone towards understanding the molecular circadian clock, the cell-autonomous transcriptional feedback loops and post-translational modifications that generate ~24 hour molecular oscillations. In Drosophila and mammals, the central circadian clocks that control rhythmicity are located in neuronal groups within the brain. Yet, little is known about how the molecular clock regulates the output of these neurons to promote rhythmicity. My lab is interested in understanding the processes that occur downstream of the molecular clock to mediate circadian neuron function. I have previously demonstrated that a putative sodium leak channel, narrow abdomen (na), is an important component of circadian neuronal output in Drosophila. We are now using this system to further characterize the function and regulation of this unique channel, with a particular interest in determining whether NA is subject to circadian regulation. In addition, my lab is utilizing the molecular and genetic tools of Drosophila in order to identify new circadian rhythms genes, with a focus on genes likely to function in circadian neuronal output.
I currently have openings in my lab for both students and postdocs.
Zhang, L., B.Y. Chung, B.C. Lear, V.L. Kilman, Y Liu, G Mahesh, R.A. Meissner, P.E. Hardin, and R. Allada. “DN1p Circadian Neurons Coordinate Acute Light and PDF Inputs to Produce Robust Daily Behavior in Drosophila
.” Current Biology 20(7): 591-599. http://dx.doi.org/10.1016/j.cub.2010.02.056
Pfeiffenberger, C., B.C. Lear, K.P. Keegan, and R. Allada. “Sleep and circadian behavior monitoring in adult Drosophila.” In: Drosophila Neurobiology: A Laboratory Manual, eds. B. Zhang, M.R. Freeman, and S. Waddell, Cold Spring Harbor Press. 483-504.
Zhang, L., B.C. Lear, A. Seluzicki, and R. Allada (2009). “The Cryptochrome photoreceptor gates PDF neuropeptide signaling to set circadian network hierarchy in Drosophila
.” Current Biology 19: 2050-2055. http://dx.doi.org/10.1016/j.cub.2009.10.058
Lear, B.C., J.M. Lin, J.R. Keath, J.J. McGill, I.M. Raman, and R. Allada (2005). “The ion channel Narrow Abdomen is critical for neural output of the Drosophila
circadian pacemaker.” Neuron 48: 965-976. http://dx.doi.org/10.1016/j.neuron.2005.10.030
Lear, B.C., C.E. Merrill, J.M. Lin, A. Schroeder, L. Zhang, and R. Allada (2005). “A G-protein coupled receptor, groom-of-PDF, is required for PDF neuron action in circadian behavior.” Neuron 48: 221-227. http://dx.doi.org/10.1016/j.neuron.2005.09.008
Fujioka, M., B.C. Lear, M. Landgraf, G.L. Yusibova, J. Zhou, K.M. Riley, N.H. Patel, and J.B. Jaynes (2003). “Even-skipped, acting as a repressor, regulates axonal projections in Drosophila
,” Development 130: 5385-5400. http://dx.doi.org/10.1242/dev.00770
Lear, B.C., J.B. Skeath, and N.H. Patel (1999). “Neural cell fate in rca1 mutants: the roles of intrinsic and extrinsic factors in asymmetric division in the Drosophila
CNS,” Mechanisms of Development 88: 207-219. http://dx.doi.org/10.1016/S0925-4773(99